National standardization of ZAP-70 determination by flow cytometry: The French experience
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چکیده
منابع مشابه
National standardization of ZAP-70 determination by flow cytometry: the French experience.
BACKGROUND ZAP-70, after being considered as a potential surrogate for VH mutational status, has seen its own prognostic value emerge. We aimed at standardizing a simple, fast, and reproducible flow cytometry method. METHODS AntiZAP-70 antibody 2F3.2 was used with indirect labeling and secondary anti-IgG2a antibody. The reference values for the expression of the results were determined on 45 ...
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BACKGROUND ZAP-70 has emerged as a potential pivotal prognostic marker for patients with chronic lymphocytic leukemia (CLL), which could replace immunoglobulin heavy chain mutation status. Although several flow cytometry assays have been described for assessing ZAP-70 in CLL, certain technical and scientific issues remain unsolved, which have prevented results of this crucial test from being re...
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BACKGROUND Flow cytometry allows specific assessment of the expression of ZAP-70, a promising new prognostic factor in B-cell chronic lymphocytic leukemia (B-CLL), but suffers from a lack of multicenter standardization. DESIGN AND METHODS An optimized method for direct detection of ZAP-70 in flow cytometry was tested in a multicenter fashion. Adapted for frozen cells, this method includes a n...
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ژورنال
عنوان ژورنال: Cytometry Part B: Clinical Cytometry
سال: 2007
ISSN: 1552-4949,1552-4957
DOI: 10.1002/cyto.b.20350